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Anonymous
2016-01-13 19:04:10 Post No. 7781370
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Anonymous
2016-01-13 19:04:10
Post No. 7781370
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If I were to have a putative gene sequence, how would I determine its protein and likely function by laboratory techniques?
Here's what I'm currently thinking:
>Isolate the specific gene of interest
>Amplify the DNA sequence
>Insert into vector and transform into an organism
>Isolate the expressed recombinant protein
>Isolate the proteins by size & MW (SDS-PAGE then MW Chromatography?)
>Determine AA sequence of protein by Western blot (?)
>DNA Microarray to determine localisation & expression of that gene sequence
>Then finally gene knockout in a species to observe function & functional gene
